Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
نویسندگان
چکیده
منابع مشابه
A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei
Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same inducible system is used to produce double-stranded RNA for RNAi knockdown of target genes. This study ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2017
ISSN: 1940-087X
DOI: 10.3791/55187